RFID as an important component technology of IoT faces important security risks while being rapidly applied, among which the discovery of unauthorized readers in space is crucial. There are some researches proposed the unauthorized reader detection algorithm based on commercial off the shell(COTS) devices, but these detection algorithms are often easily affected by moving objects blocking interference in space, causing false alarms. We propose a new method of eliminating moving object interference, which can reduce the system false alarm rate to less than 7.9% by experimental testing